Recombinant Mouse IL-2 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Mouse IL-2 Protein |
| Target Symbol | IL2 |
| Alternative Names | Interleukin 2, IL2, T Cell Growth Factor, TCGF, Aldesleukin, Lymphokine |
| Accession Number | P04351 |
| Expression System | E. coli. |
| Molecular Weight | 17.1 kDa (monomer, predicted) |
| Sequence | Ala21-Gln169, with an N-terminal Met |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using mouse CTLL-2 cells. The ED50 for this effect is typically <0.2 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.05 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Interleukin-2 (IL-2) is a cytokine primarily produced by T cells and plays a critical role in the proliferation of T cells through paracrine and autocrine activity. It further regulates immune response through promoting the differentiation of T cells into various subsets such as effector Th17 cells or regulatory T (Treg) cells. IL-2 also promotes the differentiation and stimulation of B cell and NK cell populations. The receptor for IL-2 is a heterotrimeric complex composed of IL-2 R alpha (CD25), IL-2 R beta (CD122) and IL-2 R gamma (also named, common gamma chain or CD132). IL-2 has garnered interest in cancer immunotherapy and adoptive cell therapy (ACT) where research techniques in cell culture have included IL-2 to support the expansion and survival of modified T cells in chimeric antigen receptor (CAR) T cell therapy, tumor-infiltrating lymphocyte (TIL) therapy and NK cells. |
| Tissue Specificity | IL-2 expression has been identified in lymph node, spleen, and brain. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | IL-2 dysregulation is implicated in autoimmune disorders (rheumatoid arthritis, multiple sclerosis, and type 1 diabetes), allergic asthma, allergic rhinitis, and inflammatory bowel disease (IBD). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0031 |
| Size | 100ug |
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