Custom Protein Expression (CPE) provides custom recombinant antibody production from your antibody sequence. Our service covers gene synthesis, mammalian expression, purification and quality control for antibody characterization and assay development. The standard production cycle is 12 business days from sequence confirmation, excluding shipping time.
Choose from full-length human, mouse and rabbit IgG, VHH and scFv formats, or supported bispecific designs. Share your sequence, target quantity and intended application so our technical team can review the production plan and define the delivery specifications.
Human IgG, mouse IgG and rabbit IgG
VHH-Fc and VHH-His
scFv-Fc and scFv-His
Symmetric IgG plus VHH or scFv designs; asymmetric designs based on a knobs-into-holes (KIH) system
Please include the desired isotype, Fc sequence, tags and chain architecture when submitting an engineered construct.
Mammalian expression options include CHO-K1 and Expi293F cells. Host selection depends on the antibody construct and project requirements. The representative cases below show expression in both systems.
Guaranteed purified antibody quantity by culture volume.
Each monoclonal antibody package guarantees the purified antibody quantity listed above. The selected package and QC specifications are recorded in your quotation.
Bispecific antibody production starts at 100 mL. Projects are evaluated individually, with no standard minimum delivery quantity guaranteed across bispecific constructs.
The standard production cycle covers five stages over 12 business days, starting from sequence confirmation. Shipping time is additional.
Confirm antibody sequences, format, target quantity and QC requirements.
Begin gene synthesis and preparation of the expression constructs.
Introduce the expression constructs into the selected mammalian cells and proceed with expression.
Purify the expressed antibody using a process suited to the construct.
Complete the agreed quality checks and prepare the antibody and COA for shipment.
All milestones are counted in business days. The 12-business-day production cycle excludes shipping and transit time. Please provide your required receipt date so production and shipping can be scheduled accordingly.
Each project includes purified antibody and a COA. Agree on the required quantity and release specifications before production, particularly when the antibody will be used in a sensitive functional assay.
Any required acceptance threshold is agreed for the project.
Discuss final concentration, buffer and any application-specific limits during project review so that these requirements can be assessed before work begins.
ELISA-based binding analysis and surface plasmon resonance (SPR) measurements can be discussed as additional services. Provide the target antigen and experimental objective so the testing scope can be defined. Binding or affinity measurements are separate from the standard production QC package.
Purity and endotoxin measurements do not establish target binding or biological activity. If activity is a delivery requirement, discuss the relevant assay and acceptance criteria before production.
Please provide antibody sequences, chain assignments and format, the desired quantity, intended application, required QC limits and target completion date. For bispecific antibodies, include the complete chain design and any relevant Fc or pairing modifications.
The following production partner case studies illustrate expression across conventional IgG, VHH and bispecific formats. Values are reported expression levels for individual constructs and are not guaranteed yields for other sequences.
| Antibody construct | Expression host | Reported expression |
|---|---|---|
| Human IgG1 (LALAPG), kappa | CHO-K1 | 500 mg/L |
| VHH-His | Expi293F | 220 mg/L |
| VHH-Fc | Expi293F | 300 mg/L |
| Cross mAb format | CHO-K1 | 420 mg/L |
| Bispecific IgG plus scFv | CHO-K1 | 320 mg/L |
Expression level in mg/L describes culture output. It is separate from the guaranteed purified antibody quantity for the selected monoclonal antibody package.
500mg/L
A human IgG1 (LALAPG) construct with a kappa light chain was expressed in CHO-K1 cells at a reported level of 500 mg/L. The case includes SDS-PAGE and SEC-HPLC data.
The panels show the analytical results supplied for this example. They do not establish target binding or functional activity.
220mg/L
A VHH-His construct expressed in Expi293F cells reached a reported expression level of 220 mg/L. The analytical panels below accompany this example of a tagged single-domain antibody.
320mg/L
A bispecific construct combining full-length IgG with scFv domains was expressed in CHO-K1 cells at a reported level of 320 mg/L. The case includes the analytical data below.
Case results are construct-specific. The examples support production planning and do not replace qualification in the intended downstream assay.
Please provide the antibody sequence or sequences, chain assignments, desired format, target quantity and intended application. Include Fc modifications, tags and any specific QC or timing requirements so the technical team can assess the project.
This service starts with a defined antibody sequence. If your project requires antibody generation from an antigen, please discuss our Antibody Production services for monoclonal or polyclonal antibody development.
Monoclonal antibody packages offer guaranteed purified antibody quantities of 2, 10, 20, 50 or 100 mg, using culture volumes from 30 mL to 1 L. Bispecific projects start at 100 mL and do not carry a standard minimum delivery quantity.
The standard production cycle is 12 business days from sequence confirmation to QC release, excluding shipping and transit time. Please provide your required receipt date when requesting a quotation so production and shipping can be scheduled accordingly.
The COA includes concentration, endotoxin, SDS-PAGE and SEC-HPLC data. Standard specifications are at least 90% purity by SDS-PAGE and endotoxin below 1 EU/mg. Discuss any additional release requirements during project review.
ELISA-based binding analysis and SPR measurements can be discussed as additional services. They are scoped separately from production QC and depend on the target antigen, antibody format and measurement requirements.
Share your antibody sequence, format, target quantity and intended application with CPE. Our technical team will review your requirements and discuss the expression system, QC scope and proposed schedule.
Need help defining the construct or delivery specifications? Contact us with your research objective to begin a technical discussion.
