Recombinant Mouse GM-CSF/CSF2 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Mouse GM-CSF/CSF2 Protein |
| Target Symbol | CSF2 |
| Alternative Names | Granulocyte Macrophage Growth Factor, CSF2, Sargramostim, Molgramostim, Colony Stimulating Factor 2, Pluripoietin-a, MGI-IGM, Csfgm |
| Accession Number | Q14AD9 |
| Expression System | E. coli. |
| Molecular Weight | 14.3 kDa (monomer, predicted) |
| Sequence | Ala18-Lys141, with an N-terminal Met |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using mouse FDC-P1 cells. The ED50 for this effect is typically <50 pg/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.5 EU per 1 ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Granulocyte Macrophage Growth Factor (GM-CSF), also known as CSF2, is a cytokine with diverse biological functions, primarily related to immune and inflammatory responses. In addition to a role in maintaining a niche environment for hematopoietic stems cells, GM-CSF stimulates the growth and differentiation of hematopoietic progenitor cells from various lineages including granulocytes (neutrophils, eosinophils, and basophils) and macrophages. The production of GM-CSF by T cells, macrophages, dendritic cells, B cells, eosinophils, and mast cells leads to the differentiation and activation of various immune cells, including macrophages and dendritic cells. Activation of dendritic cells and T cells involving GM-CSF plays a role in the native anti-tumor immune response, which has led to immunotherapies such as chimeric antigen receptor (CAR) T cell therapy. GM-CSF is also involved in enhancement of antibody production by B cells, the Th1 polarized immune response, and tissue repair and remodeling processes. GM-CSF binds a receptor complex composed of the GM-CSF R alpha (CD116) and common beta chain (CD131). |
| Tissue Specificity | Bone marrow stromal cells, lymph node, spleen, vascular endothelial cells, respiratory and gastrointestinal epithelial cells, and fibroblasts. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of GM-CSF production is implicated in autoimmune disorders (rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease), cancer tumor progression, cancer immune evasion, leukemia, myelodysplastic syndromes, pulmonary alveolar proteinosis (PAP), allergic inflammation (asthma, atopic dermatitis) and neuroinflammatory processes in Alzheimer’s disease and Parkinson’s disease. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0029 |
| Size | 100ug |
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