Recombinant Human VEGF 165 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human VEGF 165 Protein |
| Target Symbol | VEGFA |
| Alternative Names | Vascular Endothelial Growth Factor, VEGF-A, Vascular Permeability Factor, VPF, MVCD1 |
| Accession Number | P15692 |
| Expression System | E. coli. Disulfide-linked homodimer |
| Molecular Weight | 19.2 kDa (monomer, predicted) |
| Sequence | Ala27-Arg191 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 1-5 ng/mL. |
| Formulation | Lyophilized from sterile HEPES and NaCl with Trehalose, pH 7.4 |
| Endotoxin Level | <0.001 EU/1ug (<1 EU/mg) of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Vascular endothelial growth factor (VEGF) is involved in angiogenesis, vasculogenesis, neurogenesis and endothelial cell growth during embryonic development and in adults. It induces endothelial cell proliferation, migration, and permeabilization of blood vessels. VEGF is upregulated during hypoxia, inflammation, tissue repair and during neovascularization of tumors and adipose tissue. Additionally, VEGF induces neural progenitor cell proliferation and regulates mesenchymal stem cell differentiation. VEGF binds to the VEGFR1 (FLT1) and VEGFR2 (KDR/CD309) receptors, however, Neuropilin-1 will also bind VEGF as a coreceptor for VEGFR2. |
| Tissue Specificity | VEGF expression has been reported in vascular endothelial cells, ovaries, adipose tissue, skeletal muscle and most tumors. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | VEGF dysregulation is implicated in macular degeneration, diabetic retinopathy, rheumatoid arthritis, psoriasis, and cancer. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0028UL |
| Size | 100ug |
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