Recombinant Human TNF-alpha Protein, Animal-Free, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human TNF-alpha Protein, Animal-Free |
| Target Symbol | TNF |
| Alternative Names | Tumor Necrosis Factor alpha, TNF, TNFA, APC1, TNFSF2, Tumor Necrosis Factor Ligand Superfamily Member 2, Differentiation Inducing Factor, DIF, Cachectin |
| Accession Number | P01375.1 |
| Expression System | E. coli using an animal component-free system. |
| Molecular Weight | 17.4 kDa (monomer, predicted) |
| Sequence | Val77-Leu233 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by a cell proliferation assay using mouse L-929 cells treated with Actinomycin D. The ED50 for this effect is typically <40 pg/mL. |
| Formulation | Lyophilized from sterile phosphate buffer with Trehalose, pH 8.0 |
| Endotoxin Level | <0.001 EU per 1 μg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. Optionally, use PBS with 0.1% BSA. |
| Target Function | Tumor Necrosis Factor-alpha (TNF-alpha) was initially associated as a proinflammatory cytokine expressed by macrophages. However, TNF-alpha also regulates apoptosis, tumor cell death, lipid metabolism and cell differentiation of T cells, B cells and NK cells. There are 2 receptors for TNF-alpha, a ubiquitously expressed TNFR1 (CD120a) homotrimer containing an apoptotic pathway trigger and the TNFR2 (CD120b) homotrimer that is commonly restricted to epithelial, endothelial and specific immune cells. |
| Tissue Specificity | TNF-alpha expression has been identified in adipose tissue, microglia, fibroblasts, endothelia tissue, and epithelial tissue. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms |
| Involvement In Disease | Dysregulation of TNF-alpha is implicated in rheumatoid arthritis, inflammatory bowel disease (IBD), psoriasis, ankylosing spondylitis, systemic lupus erythematosus (SLE), systemic sclerosis (SSc), multiple sclerosis (MS) and insulin resistance in type 2 diabetes |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0046UL |
| Size | 100ug |
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