Recombinant Human Thrombopoietin/TPO Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human Thrombopoietin/TPO Protein |
| Target Symbol | THPO |
| Alternative Names | Thrombopoietin, THPO, TPO, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth And Development Factor, Megakaryocyte Colony-Stimulating Factor, Thrombopoietin Nirs, C-Mpl Ligand, MPLLG, MGDF, THCYT1, CAMT2, MKCSF |
| Accession Number | P40225 |
| Expression System | CHO cell line |
| Molecular Weight | 18.7 kDa (monomer, predicted), 20 kDa and 18 kDa (glycosylated, observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively) |
| Sequence | Ser22-Leu195 |
| Purity | >95% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using MO7e human cells. The ED50 for this effect is typically <10 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.1 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Thrombopoietin (TPO) plays an important role in the proliferation and maturation of megakaryocytes and their progenitors in regulating platelet production. TPO has a role in promoting the survival, self-renewal, and expansion of hematopoietic stem cells, along with primitive multilineage progenitor cells. This has led to further interest in applications for in vitro and ex vivo maintenance, expansion and differentiation of hematopoietic stem cells. TPO is the ligand for c-Mpl (CD110). |
| Tissue Specificity | TPO is primarily synthesized in the liver, and to lesser degrees in the kidneys, bone marrow and spleen. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of TPO is associated with idiopathic thrombocytopenic purpura (ITP), aplastic anemia, myelodysplastic syndromes (MDS), thrombocytosis, hepatitis, cirrhosis, and chemotherapy-induced thrombocytopenia. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0027 |
| Size | 100ug |
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