Recombinant Human Soluble DLK-1 (sDLK-1) Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human Soluble DLK-1 (sDLK-1) Protein |
| Target Symbol | DLK1 |
| Alternative Names | FA1 (Fetal Antigen 1) |
| Accession Number | P80370.3 |
| Expression System | HEK293 cell line |
| Molecular Weight | 29.9 kDa (monomer, predicted), 40-45 kDa (observed) |
| Sequence | Ala24-Ala304 |
| Purity | >98% under reducing conditions by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by an inhibition of adipogenesis differentiation using 3T3-L1 cells (Smas, C.M. et al. (1993) Cell73:725). There is >40% inhibition of differentiation as measured by Oil Red O staining with 10-100 ng/mL sDLK-1 treatment. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.001 EU per 1 μg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. Optionally, use PBS with 0.1% BSA. |
| Target Function | The soluble form of human Delta-like 1 homolog (sDLK-1) is a circulating glycoprotein that primarily acts as a master regulator of cell differentiation and metabolic adaptation. sDLK-1 generally functions to maintain stem and progenitor cells in an undifferentiated, proliferative state. It is a well-characterized inhibitor of several mesodermal differentiation pathways. sDLK-1 functions as an endocrine signaling molecule, particularly during periods of high metabolic demand like pregnancy or early development. Unlike its typical inhibitory roles, soluble DLK-1 has been shown to stimulate angiogenesis (the formation of new blood vessels) by activating Notch1/Akt signaling in endothelial cells. In certain contexts, such as pancreatic cancer models, sDLK-1 exhibits antitumor effects by suppressing cell migration, wound healing, and colony formation. Because it is highly expressed in the fetus and placenta, maternal serum levels of sDLK-1 are used as a marker for fetal growth restriction and other pregnancy complications. |
| Tissue Specificity | Expression in the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | It acts as a paracrine/endocrine regulator, with significant involvement in various diseases, particularly in bone metabolism, obesity, cancer, and cardiovascular fibrosis |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0008UL |
| Size | 100ug |
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