Recombinant Human SCF Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human SCF Protein |
| Target Symbol | KITLG |
| Alternative Names | Stem Cell Factor, SCF, Mast Cell Growth Factor, DFNA69, Kit Ligand, KITLG, C-Kit Ligand, MGF, Steel Factor, Kitl, KL-1, FPH2, SLF, SHEP7, DCUA, FPHH, WS2F |
| Accession Number | P21583 |
| Expression System | E. coli. |
| Molecular Weight | 18.6 kDa (predicted) |
| Sequence | Glu26-Ala189, with an N-terminal Met |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by a cell proliferation assay using human TF-1 cells. The ED50 for this effect is typically <8 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.005 EU per 1 µg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. If desired, 5% Trehalose or 0.1% BSA may be added. |
| Target Function | Stem Cell Factor (SCF) plays an important role acting on progenitor cells in hematopoiesis, germ cell development, and melanogenesis. SCF not only has a role in expansion and differentiation of stem cells but is an important factor in preventing apoptosis and promoting self-renewal and survival of stem cells. The receptor for SCF is c-KIT (CD117). c-Kit expression is lost during differentiation of hematopoietic cells, with the exception of mast cells. SCF induces mast cell proliferation and histamine release. |
| Tissue Specificity | SCF expression has been reported in skin (particularly in basal layer of the epidermis), stomach, intestines, testes, ovaries, brain, thymus and lymph nodes. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms |
| Involvement In Disease | Dysregulation of SCF has been implicated in gastrointestinal stromal tumors (GISTs), leukemia, melanomas, breast cancer, aplastic anemia, myelodysplastic syndromes, mastocytosis, pigmentation disorders (piebaldism and Waardenburg syndrome), gonadal dysgenesis, inflammatory bowel disease (IBD), Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, and allergic asthma. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0045UL |
| Size | 100ug |
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