Recombinant Human R-Spondin 3/RSPO3 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human R-Spondin 3/RSPO3 Protein |
| Target Symbol | RSPO3 |
| Alternative Names | Roof Plate-specific Spondin 3, RSPO3, Thrombospondin Type I Domain Containing 2, Protein With TSP Type-1 Repeat, THSD2, PWTSR, CRISTIN1, HPWTSR, HRspo3, FLJ14440 |
| Accession Number | Q9BXY4 |
| Expression System | Mammalian cell line. |
| Molecular Weight | 28.2 kDa (predicted) |
| Sequence | Asn23-His272 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by the ability to promote proliferation of neuroblastoma cells.The ED50 for this effect is typically <40 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.005 EU per 1 μg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Roof plate-specific Spondin 3 (R-Spondin 3 or RSPO3) is a key regulator of Wnt signaling with diverse functions in during embryonic development, tissue homeostasis, and stem cell maintenance. In embryonic development, RSPO3 is involved in various processes, including limb bud development, skeletal patterning, and organogenesis. In adults, it plays a role in maintaining tissue homeostasis, particularly in stem cell niches where Wnt signaling is critical for the regulation of stem cell self-renewal and differentiation. RSPO3 enhances Wnt signaling by competing with Wnt antagonist DKK-1 for binding to Low-Density Lipoprotein Receptor-related Proteins LRP4, LRP5 or LRP6 and inhibiting its interaction with the co-receptor Kremen. This inhibition leads to the stabilization of the Frizzled/LRP receptor complex, which initiates downstream signaling events mediated by Wnt proteins. As the in vivo roles of RSPO3 have been elucidated in tissue patterning and stem cell maintenance, RSPO3 plays a more common role for in vitro cell culture and the growth and maintenance of 3-D organoid cultures. |
| Tissue Specificity | R-Spondin 3 expression has been identified in limb buds, neural tube, Intestinal crypt epithelial cells, mammary glands, lung epithelium, liver bile ducts, basal layer of epidermis, hair follicles, ovaries and testes. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of R-Spondin 3 is associated with autosomal dominant polycystic kidney disease (ADPKD), brachydactyly type B1 (BDB1), Tetra-amelia syndrome, aplastic anemia and thrombocytopenia (TAAD), osteoarthritic cartilage, and numerous cancers (colorectal, gastric, breast, ovarian, liver and prostate). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0049UL |
| Size | 100ug |
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