Recombinant Human R-Spondin 1/RSPO1 Protein, Active
Description
Product Overview
| Product Name | Recombinant Human R-Spondin 1/RSPO1 Protein |
| Target Symbol | RSPO1 |
| Alternative Names | Roof Plate-specific Spondin 1, RSPO1, CRISTIN3, R-Spondin Homolog, HRspo1, RSPO, FLJ40906 |
| Accession Number | Q2MKA7 |
| Expression System | Mammalian cell line. |
| Molecular Weight | 26.9 kDa (predicted) |
| Sequence | Ser21-Ala263, with an N-terminal Met |
| Purity | Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by in-house activity assay |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | Available Upon Request |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Roof Plate-specific Spondin 1 (R-Spondin 1 or RSPO1) is a key regulator of Wnt signaling with diverse functions in during embryonic development, tissue homeostasis, and stem cell maintenance. Its precise role depends on the cellular context and interactions with other signaling pathways. RSPO1 enhances Wnt signaling by competing with Wnt antagonist DKK-1 for binding to LRP6 (Low-Density Lipoprotein Receptor-related Protein 6) and inhibiting its interaction with the co-receptor Kremen. This inhibition leads to the stabilization of the Frizzled/LRP6 receptor complex, which initiates downstream signaling events mediated by Wnt proteins. The role in tissue patterning is observed in expression of RSPO1 in the roof plate boundary during early development leading to involvement in organogenesis and gonadal development of both ovaries and testes. As the in vivo roles of RSPO1 have been elucidated in tissue patterning and stem cell maintenance, RSPO1 plays a more common role for in vitro cell culture and the growth and maintenance of 3-D organoid cultures. |
| Tissue Specificity | Intestinal crypt epithelial cells, kidney epithelial cells, basal layer of epidermis, hair follicles, and prostate tissue. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of RSPO1 expression is implicated in various cancers due to enhanced Wnt signaling and the resultant cell proliferation and metastasis of tumors, as well as palmoplantar hyperkeratosis. Rare genetic mutations in the RSPO1 gene are linked to polydactyly, syndactyly and XX disorders of sex development with atypical gonadal development. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0044 |
| Size | 100ug |
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