Recombinant Human PDGF-BB Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human PDGF-BB Protein |
| Target Symbol | PDGFB |
| Alternative Names | Platelet Derived Growth Factor BB, PDGFBB, Platelet-Derived Growth Factor B, PDGFB, PDGF Subunit B, Epididymis Secretory Sperm Binding Protein, Oncogene SIS, SIS, Proto-Oncogene C-SIS, C-SIS, Becaplermin |
| Accession Number | P01127.1 |
| Expression System | E. coli. Disulfide-linked homodimer |
| Molecular Weight | 12.3 kDa (monomer, predicted), 13 kDa and 28 kDa (observed on SDS-PAGE gel stained with Coomassie® Blue under reducing and non-reducing conditions, respectively). |
| Sequence | Ser82-Thr190 |
| Purity | >96% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by a cell proliferation assay using mouse Balb/c 3T3 cells. The ED50 for this effect is typically 1-3 ng/mL |
| Formulation | Lyophilized from sterile Sodium Acetate and NaCl with Trehalose, without carrier protein. |
| Endotoxin Level | <0.2 EU per 1 µg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile 4 mM HCl. |
| Target Function | Platelet-Derived Growth Factor BB (PDGF-BB) is a protein with roles in regulating of many processes including embryonic development, angiogenesis, cell proliferation and differentiation. PDGF-BB acts on mesenchymal cells as a mitogen for connective tissue formation, particularly fibroblasts, vascular smooth muscle cells and glial cells. PDGF-BB is present in the alpha granules of platelets. As such, PDGF-BB promotes angiogenesis, chemotaxis of fibroblasts and collagen synthesis in tissue repair. Additionally, PDGF-BB supports development of kidney glomeruli, the differentiation of neural stem cells and has neuroprotective functions on mature neurons during injury. PDGF-BB primarily binds to the PDGFR beta (CD140B) homodimer, and with lower affinity to the PDGFR alpha (CD140a) homodimer or PDGFR alpha/PDGFR beta heterodimer leading to activation of the MAPK, PI3K, and STAT3 pathways. |
| Tissue Specificity | PDGF-BB expression has been identified in endothelial tissue, smooth muscle tissue, heart, lung, brain, and neurons. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of PDGF-BB is associated with atherosclerosis, pulmonary hypertension, glomerulonephritis, diabetic retinopathy, keloids, hypertrophic scars, cancer (glioma, sarcoma, and tumor angiogenesis). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0024 |
| Size | 100ug |
Need a different construct, tag or scale?
Our custom recombinant protein production service covers gene synthesis, expression in E. coli, yeast, insect and mammalian cells, purification and QC, for Non-GMP research or GMP manufacturing.