Recombinant Human PDGF-AA Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human PDGF-AA Protein |
| Target Symbol | PDGFA |
| Alternative Names | Platelet-derived Growth Factor AA, Platelet Derived Growth Factor Subunit A, Platelet Derived Growth Factor A Chain, Platelet Derived Growth Factor Alpha Chain, Platelet Derived Growth Factor Alpha Polypeptide, PDGF Subunit A, PDGF A-Chain, PDGF1 |
| Accession Number | P04085 |
| Expression System | E. coli. |
| Molecular Weight | 14.3 kDa (monomer, predicted), 14 kDa and 29 kDa observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively |
| Sequence | Ser87-Thr211 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by a cell proliferation assay using mouse BALB/c 3T3 cells. The ED50 for this effect is typically <25 ng/mL |
| Formulation | Lyophilized from sterile Sodium Acetate and NaCl with Trehalose, without carrier protein. |
| Endotoxin Level | <0.02 EU per 1 μg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Platelet-Derived Growth Factor AA (PDGF-AA) is a protein with roles in regulating of many processes including embryonic development, angiogenesis, cell proliferation and differentiation. PDGF-BB acts on mesenchymal cells as a mitogen for connective tissue formation, particularly fibroblasts, vascular smooth muscle cells and glial cells. PDGF-AA is present in the alpha granules of platelets. As such, PDGF-AA promotes angiogenesis, chemotaxis of fibroblasts and collagen synthesis in tissue repair. Additionally, PDGF-AA supports development of kidney glomeruli, the differentiation of neural stem cells and has neuroprotective functions on mature neurons during injury. PDGF-AA binds to the PDGFR alpha (CD140a) homodimer only and activates the MAPK, PI3K, and STAT3 pathways. |
| Tissue Specificity | PDGF-AA expression has been identified in epithelial tissue, endothelial tissue, smooth muscle tissue, colon, heart, lung, brain and neurons. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of PDGF-AA is associated with atherosclerosis, pulmonary hypertension, glomerulonephritis, diabetic retinopathy, keloids, hypertrophic scars, cancer (glioma, sarcoma, and tumor angiogenesis). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0058UL |
| Size | 100ug |
Need a different construct, tag or scale?
Our custom recombinant protein production service covers gene synthesis, expression in E. coli, yeast, insect and mammalian cells, purification and QC, for Non-GMP research or GMP manufacturing.