Recombinant Human Neurotrophin 3/NT-3 Protein, Active
Description
Product Overview
| Product Name | Recombinant Human Neurotrophin 3/NT-3 Protein |
| Target Symbol | NTF3 |
| Alternative Names | Neurotrophin 3, NT3, NTF3, Nerve Growth Factor 2, NGF2, HDNF |
| Accession Number | P20783 |
| Expression System | E. coli. |
| Molecular Weight | 13.8 kDa (predicted) |
| Sequence | Tyr149-Thr257 |
| Purity | Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by in-house activity assay |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | Available Upon Request |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Neurotrophin 3 (NT-3) belongs to the neurotrophin family and primarily functions as a growth factor that promotes the survival, growth, and differentiation of neurons and oligodendrocytes. NT-3 stimulates the growth and branching of axons and dendrites. This process is crucial for the establishment of neuronal circuits during development and for the regeneration of neural connections after injury. NT-3 enhances neuroprotection and regulates synaptic plasticity in mature neurons. Outside of the CNS, NT-3 promotes differentiation and survival of peripheral, motor, and sensory neurons. NT-3 primarily binds to TrkC as the high affinity signaling receptor, but association with TrkB and the low-affinity p75-NTR has been reported. In cell culture, NT-3 has been used to maintain neuronal cells in culture, the generation of peripheral neurons and oligodendrocyte precursors. It has also been used to differentiate human pluripotent stem cells (hPSCs) to glutamatergic and GABAergic neurons and the culture of 3-D organoids. |
| Tissue Specificity | NT-3 expression has been identified in brain (cerebral cortex, hippocampus, cerebellum), spinal cord, peripheral nerves, ganglia, and to a lesser extent in skin, skeletal muscles, heart, kidney, and liver. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of NT-3 is associated with Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, diabetic neuropathy, Charcot-Marie-Tooth disease, depression, anxiety, amyotrophic lateral sclerosis (ALS), and muscular dystrophies. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0057 |
| Size | 100ug |
Need a different construct, tag or scale?
Our custom recombinant protein production service covers gene synthesis, expression in E. coli, yeast, insect and mammalian cells, purification and QC, for Non-GMP research or GMP manufacturing.