Recombinant Human/Mouse/Rat Activin A Protein , Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human/Mouse/Rat Activin A Protein |
| Target Symbol | INHBA |
| Alternative Names | Inhibin Subunit Beta A, Erythroid Differentiation Factor, Inhibin Beta A Chain, Activin Beta-A Chain, Follicle-Stimulating Hormone-Releasing Protein, FSH-Releasing Protein, INHBA, EFD, FRP |
| Accession Number | P08476 |
| Expression System | E. coli. |
| Molecular Weight | 13 kDa (monomer, predicted) |
| Sequence | Gly311-Ser426 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by a cell proliferation assay using mouse L-929 cells treated with Actinomycin D. The ED50 for this effect is typically <40 pg/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.001 EU per 1 μg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Activin A plays critical roles in various physiological processes, including development, reproduction, immune function, tissue repair and tissue homeostasis. During embryonic development, Activin A is involved in patterning and differentiation of various tissues and organs. It helps regulate processes such as mesoderm induction, neural differentiation, and organogenesis. Activin A is involved in the regulation of reproductive processes such as follicle development in the ovaries, spermatogenesis in the testes, and embryonic implantation in the uterus. It also plays a role in the secretion of hormones such as follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary gland. Activin A has immunomodulatory effects, influencing the function of immune cells such as macrophages, T cells, and B cells. Activin A participates in the regulation of bone metabolism by influencing the activity of osteoblasts and osteoclasts. During tissue repair, Activin A is upregulated and promotes growth of keratinocytes and stromal cells. Activin A is a homodimer consisting of two betaA subunits. Activin A signaling takes places through binding to a Type II receptor (Activin RIIA/ACVR2A or Activin RIIB/ACVR2B) followed by heterodimerization with a Type I receptor unit (Activin RIB/ACVR1B/ALK-4 or Activin RIC/ACVR1C/ALK-7). Regulation of Activin A occurs by binding to inhibitory proteins (Follistatin and FLRG) or membrane-bound decoy receptors (BAMBI and Cripto). Activin A is used in cell culture to maintain pluripotency of human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) and to differentiate human ESCs into endoderm. Similarly, Activin A is used for the maintenance and growth of 3-D organoids. |
| Tissue Specificity | Activin A expression has been reported in adipose tissue, ovaries, testes, uterus, pituitary gland, placenta, prostate, lung, kidney, skeletal muscle, and cardiac muscle. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of Activin A has been associated with osteoporosis, rheumatoid arthritis (RA), inflammatory bowel disease (IBD), asthma, obesity, type 2 diabetes, atherosclerosis, and cancers (prostate, breast, ovarian, and colorectal). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0047UL |
| Size | 100ug |
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