Recombinant Human M-CSF/CSF1 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human M-CSF/CSF1 Protein |
| Target Symbol | CSF1 |
| Alternative Names | Macrophage Colony Stimulating Factor, CSF1, CSF-1, MCSF, MGC31930, Lanimostim, PG-M-CSF, Proteoglycan Macrophage Colony Stimulating Factor |
| Accession Number | P09603 |
| Expression System | CHO cell line. Disulfide-linked homodimer |
| Molecular Weight | 25.1 kDa (monomer, predicted), 43 kDa and 86 kDa (glycosylated, observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively) |
| Sequence | Glu33-Arg255 |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using mouse M-NFS-60 cells. The ED50 for this effect is typically 2-5 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.02 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Macrophage Colony-stimulating Factor (M-CSF), also known as CSF-1, is a cytokine with roles in the growth, differentiation, and survival of monocytes and macrophages. M-CSF promotes the differentiation of monocytes into mature tissue-resident macrophages involved in the phagocytosis of pathogens, clearance of debris, and secretion of growth factors for tissue repair. In addition, M-CSF is involved in regulating bone homeostasis by stimulating osteoclast differentiation and activity. M-CSF is primarily produced by monocytes and macrophages themselves. It acts in an autocrine and paracrine manner to regulate the proliferation, differentiation, and survival of these cells. However, M-CSF is also produced in bone marrow stromal cells, fibroblasts and endothelial cells as a component in the local microenvironmental regulation of the immune cell function. M-CSF is often highly expressed in the tumor microenvironment where it recruits tumor-associated macrophages (TAMs) that suppress the native immune response leading to tumor progression and infiltration. M-CSF binds M-CSF R (CD115), a Receptor Tyrosine Kinase (RTK) family III member. |
| Tissue Specificity | Bone marrow stromal cells, spleen, vascular endothelial cells, endometrial secretory epithelium, respiratory and gastrointestinal epithelial cells, and synovial fibroblasts. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms |
| Involvement In Disease | Dysregulation of M-CSF is implicated in autoimmune disorders (rheumatoid arthritis, atherosclerosis, inflammatory bowel disease), osteoporosis, cancer tumor progression, cancer immune evasion, monocytic leukemia and neuroinflammatory processes in Alzheimer’s disease and multiple sclerosis. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0017UL |
| Size | 100ug |
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