Recombinant Human KGF/FGF-7 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human KGF/FGF-7 Protein |
| Target Symbol | FGF7 |
| Alternative Names | Keratinocyte Growth Factor, Fibroblast Growth Factor 7, KGF, FGF7, Heparin-Binding Growth Factor 7, HBGF-7 |
| Accession Number | P21781 |
| Expression System | E.Coli. |
| Molecular Weight | 19 kDa (monomer, predicted) |
| Sequence | Cys32-Thr194, with an N-terminal Met |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by a cell proliferation assay using 4MBr-5 rhesus monkey cells. The ED50 for this effect is typically < 3 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.001 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF-7) plays a role in embryonic development, cell proliferation, tissue repair, branching morphogenesis, and tumor progression. KGF is primarily expressed in mesenchymal cells, specifically in tissues where epithelial-mesenchymal interactions occur. As such, KGF activity is mainly exhibited on epithelial cells, particularly keratinocytes, by influencing the repair and regeneration of damaged epithelial tissues, facilitating wound healing processes and promoting pathways to protect epithelial cells from UV radiation and toxins. Additionally, KGF is involved in the development and maintenance of hair follicles, promoting hair growth. In cell culture, KGF has efficacy in the differentiation of pluripotent stem cells (PSCs) toward thymic epithelial cells and in the culture of 3-D organoids. The receptor KGF is FGFR2IIIb. |
| Tissue Specificity | KGF has been detected in mesenchymal cells in skin, lung, intestine, salivary glands, and bladder. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of KGF is associated with psoriasis, gingival hyperplasia, idiopathic pulmonary fibrosis (IPF), inflammatory bowel disease (IBD), mucositis, interstitial cystitis, and tumorigenesis in stomach, colon, pancreas, and ovarian cancers. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0016UL |
| Size | 100ug |
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