Recombinant Human IL-7 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human IL-7 Protein |
| Target Symbol | IL7 |
| Alternative Names | Interleukin 7, IL7 |
| Accession Number | P13232 |
| Expression System | E.Coli. |
| Molecular Weight | 17.5 kDa (predicted) |
| Sequence | Asp26-His177, with an N-terminal Met |
| Purity | >98% by SDS-PAGE and quantitative densitometry by CoomassiePurity ® Blue staining |
| Activity | Determined by a cell proliferation assay using 2E8 mouse cells. The ED50 for this effect is typically <0.75 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.05 EU per 1 μg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. If desired, 5% Trehalose or 0.1% BSA may be added. |
| Target Function | Interleukin 7 (IL-7) functions in regulating hematopoeitic stem cell differentiation into lymphoid progenitor cells. IL-7 also plays a role in the proliferation and survival of B cells, T cells and NK cells in the lymphoid lineage. This is led to further developments in applying IL-7 in cell culture and manufacturing strategies in cancer immunotherapy approaches, such as adoptive cell therapy (ACT) and chimeric antigen receptor (CAR) T cell therapy. |
| Tissue Specificity | IL-7 is primarily produced by stromal cells in the bone marrow and thymus with expression also reported in lymph nodes, spleen, intestinal goblet cells, keratinocytes, lung epithelium and liver sinusoidal endothelial cells. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of IL-7 has been implicated in autoimmune diseases (rheumatoid arthritis, multiple sclerosis, Crohn’s disease, ulcerative colitis), aplastic anemia myelodysplastic syndromes, asthma and chronic obstructive pulmonary disease (COPD), Graft-versus-Host Disease (GVHD), and hematological malignancies (acute lymphoblastic leukemia, T cell lymphoma). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0041 |
| Size | 100ug |
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