Recombinant Human IL-3 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human IL-3 Protein |
| Target Symbol | IL3 |
| Alternative Names | Interleukin 3, IL3, Mast Cell Growth Factor, Multilineage Colony Stimulating Factor, Multipotential Colony Stimulating Factor, Hematopoietic Growth Factor, P-Cell Stimulating Factor, MULTI-CSF, MCGF, MGC79398, MGC79399 |
| Accession Number | AAC08706 |
| Expression System | E.Coli. |
| Molecular Weight | 15 kDa (predicted) |
| Sequence | Ala20-Phe152 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by a cell proliferation assay using TF-1 human cells. The ED50 for this effect is typically <0.4 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.01 EU per 1 µg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Interleukin-3 (IL-3) is a pleiotropic cytokine that plays a crucial role in the proliferation, differentiation, and survival of hematopoietic stem cells and myeloid progenitor cells. In particular, IL-3 regulates the development of basophils, dendritic cells, eosinophils, erythrocytes, macrophages, mast cells, megakaryocytes, monocytes, and neutrophils. IL-3 is produced by a variety of cells, including activated T cells, B cells, mast cells, and vascular endothelial cells. Signaling takes place through binding to IL-3R alpha (CD123) that heterodimerizes with the common beta chain (CD131). |
| Tissue Specificity | IL-3 expression has been identified in bone marrow stromal cells and vascular endothelial tissue. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of IL-3 is associated with asthma, allergic rhinitis, atopic dermatitis, acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), aplastic anemia, multiple sclerosis (MS), rheumatoid arthritis, systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), psoriasis, rheumatoid arthritis, and myelodysplastic syndromes (MDS). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0023UL |
| Size | 100ug |
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