Recombinant Human IL-1 beta/IL-1F2 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human IL-1 beta/IL-1F2 Protein |
| Target Symbol | IL1B |
| Alternative Names | Interleukin 1 beta, IL1B, IL1F2, Catabolin, IFN beta Inducing Factor, Osteoclast Activating Factor, OAF |
| Accession Number | P01584 |
| Expression System | E.Coli. |
| Molecular Weight | 17.3 kDa (predicted) |
| Sequence | Ala117-Ser269 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically <10 pg/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.05 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Interleukin-1 beta (IL-1 beta) is a pro-inflammatory cytokine that mediates inflammation and triggers other cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). IL-1 beta helps regulate the immune response by activating immune cells such as T cells (Th1 and Th17), B cells, and macrophages. It enhances the antigen-presenting function of antigen-presenting cells (APCs). IL-1 beta has pyrogenic effects triggered through the hypothalamus. In cell culture, IL-1 beta maintains the survival of mesenchymal stromal cells and hematopoietic progenitor cells and stimulates the differentiation of mesenchymal stem cells and neural stem cells. IL-1beta signaling is triggered by binding to IL-1RI following by association with the accessory protein, IL-1 RAcP. IL-1 beta signaling is modulated by binding to two decoy receptors a soluble IL-1Ra or membrane-bound IL-1RII. |
| Tissue Specificity | IL-1 beta expression has been identified in endothelial tissue, skin, lungs, gastrointestinal tissue, microglia, astrocytes, neurons, and adipose tissue. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of IL-1 beta is associated with inflammatory disorders (Cryopyrin-Associated Periodic Syndromes (CAPS), Neonatal-Onset Multisystem Inflammatory Disease (NOMID)), autoimmune disorders (Rheumatoid Arthritis (RA), Systemic Lupus Erythematosus (SLE), Inflammatory Bowel Disease (IBD)), osteoarthritis, Juvenile Idiopathic Arthritis (JIA), psoriasis, dermatitis, Alzheimer’s Disease, Parkinson’s Disease, Multiple Sclerosis (MS), Type 2 Diabetes, Obesity, myocardial infarction, atherosclerosis and cancers by promoting inflammation in the tumor microenvironment and metastasis. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0013 |
| Size | 100ug |
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