Recombinant Human IFN-gamma Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human IFN-gamma Protein |
| Target Symbol | IFNG |
| Alternative Names | Interferon gamma, IFNG, Immune Interferon, IFG, IFI |
| Accession Number | CAA31639 |
| Expression System | E.Coli. |
| Molecular Weight | 16.9 kDa (predicted) |
| Sequence | Gln24-Gln166, with an N-terminal Met |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Activity | Determined by the ability to inhibit proliferation of HT-29 human cells. The ED50 for this effect is typically <0.75 ng/mL |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.005 EU per 1 µg of the protein as determined by the LAL method |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile, deionized water. |
| Target Function | Interferon-gamma (IFN-gamma) is a protein with predominant roles in the innate and adaptive immune response. It is secreted by Th1 cells, cytotoxic T cells, macrophages, dendritic cells, mucosal epithelial cells and NK cells. IFN-gamma promotes the differentiation of T cells into Th1 cells. Th1 cells produce IFN-gamma themselves, creating a positive feedback loop that amplifies the immune response. In addition, IFN-gamma upregulates monocytes, macrophages, and dendritic cells while increasing MHC class I and II expression. B Cell immunoglobulin class switching is also influenced by IFN-gamma. IFN-gamma RI (CD119/IFNGR1) is the ligand-binding subunit that heterodimerizes with the IFN-gamma RII (IFNGR2). |
| Tissue Specificity | IFN-gamma expression has been identified in vascular endothelial cells, respiratory and gastrointestinal epithelial cells. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of IFN-gamma is associated with autoimmune disorders (multiple sclerosis (MS), rheumatoid arthritis (RA), type 1 diabetes, and inflammatory bowel diseases (IBD)), allergic asthma, atherosclerosis, hemophagocytic lymphohistiocytosis (HLH), Alzheimer’s disease, Parkinson’s disease and multiple sclerosis (MS). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0021UL |
| Size | 100ug |
Need a different construct, tag or scale?
Our custom recombinant protein production service covers gene synthesis, expression in E. coli, yeast, insect and mammalian cells, purification and QC, for Non-GMP research or GMP manufacturing.