Recombinant Human Hyper IL-6 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human Hyper IL-6 Protein |
| Target Symbol | IL6R |
| Alternative Names | Interleukin 6, Interferon Beta-2, IFN-Beta-2, IFNB2, B-Cell Stimulatory Factor 2, BSF2, Hybridoma Growth Factor, HGF, HSF, CTL Differentiation Factor, CDF |
| Accession Number | P08887 (IL-6 R) & P05231 (IL-6) |
| Expression System | CHO cell line |
| Molecular Weight | 45.9 kDa (predicted), 42 kDa (observed on SDS-PAGE gel stained with Coomassie® Blue) |
| Sequence | sIL6R (Val112-Ala323)-GSL-IL6（Val30-Met212） |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | This IL-6/IL-6R fusion protein stimulates proliferation in DS-1 cells (IL-6R+), albeit less than IL-6 alone. Also, IL-6/IL-6R fusion protein inhibits the proliferation of HUVECs (IL-6R-). Determined by a cell proliferation assay using DS-1 mouse lymphoblast cells, the ED50 for this effect is typically <750 pg/mL. Also determined by an inhibition of proliferation assay using HUVECs, the ED50 for this effect is typically 1-10 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.005 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. If desired, 5% Trehalose or 0.1% BSA may be added. |
| Target Function | IL-6 plays a role in regulating immune response, inflammation, hematopoiesis, metabolism, and neurological processes. IL-6 influences the growth and differentiation of hematopoietic progenitor and stem cells. In the innate immune response, IL-6 is released by macrophages and dendritic cells. During the adaptive immune response, IL-6 plays a role in B cell maturation and differentiation of T cells into T follicular helper (Tfh) cells and Th17 cells. Muscle tissue and the central nervous system response to IL-6 has been linked to lipolysis and modulation of insulin response. IL-6 signaling occurs via binding to IL-6R alpha (CD126) and subsequent dimerization with the signal-transducing gp130 subunit. |
| Tissue Specificity | IL-6 expression has been reported in muscle tissue, endothelial tissue, lung, intestine, and adipose tissue. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of IL-6 is implicated in rheumatoid arthritis, systemic juvenile idiopathic arthritis (sJIA) inflammatory bowel disease (IBD), autoimmune hepatitis, atherosclerosis, insulin resistance, type 2 diabetes, fatty liver disease, obesity-related inflammation, Castleman disease, Behçet’s disease Alzheimer’s disease, systemic lupus erythematosus, multiple myeloma, and various cancers (prostate, breast, and lung) through actions on the tumor microenvironment. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0002UL |
| Size | 100ug |
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