Recombinant Human HGF Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human HGF Protein |
| Target Symbol | HGF |
| Alternative Names | Hepatocyte Growth Factor, HGFB, Scatter Factor, SF, Hepatopoietin-A, HPTA, Fibroblast-Derived Tumor Cytotoxic Factor, F-TCF, Lung Fibroblast-Derived Mitogen, DFNB39 |
| Accession Number | P14210 |
| Expression System | Expressed in HEK cells. Disulfide-linked heterodimer. |
| Molecular Weight | 53.4 kDa (alpha chain, predicted), 26 kDa (beta chain, predicted).The glycosylated 56-60 kDa (alpha chain) and 33-35 kDa (beta chain) were observed on SDS-PAGE gel stained using Coomassie® Blue under reducing conditions. |
| Sequence | Alpha chain: Lys34-Arg494 Beta chain: Val495-Ser728 |
| Purity | >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using 4MBr-5 cells. The ED50 for this effect is typically 20-50 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.005 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Hepatocyte Growth Factor (HGF) is a protein with roles in cell growth, motility of cardiomyocytes, branching morphogenesis, angiogenesis and tubulogenesis of blood vessels. It is produced by mesenchymal cells and acts primarily upon epithelial cells, endothelial cells, hematopoietic progenitors, and T cells, exerting its effects through a high-affinity receptor known as the c-Met receptor (HGF R). HGF is expressed as a preprotein that is cleaved to form the alpha and beta chains that create the mature heterodimer. |
| Tissue Specificity | HGF expression has been identified in liver, kidney, lung, stomach, intestinal tissue, pancreatic tissue, brain, skin and blood vessels. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of HGF is associated with liver cancer, lung cancer, breast cancer, gastric cancer, pancreatic cancer, liver fibrosis, cirrhosis, renal fibrosis, pulmonary fibrosis, chronic obstructive pulmonary disease (COPD), Alzheimer’s disease, Parkinson’s disease, ischemic heart disease, peripheral artery disease, atherosclerosis, and inflammatory bowel disease (IBD). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0012UL |
| Size | 100ug |
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