Recombinant Human GDNF Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human GDNF Protein |
| Target Symbol | GDNF |
| Alternative Names | Glial Cell Line Derived Growth Factor, Glial Derived Neurotrophic Factor, Astrocyte-Derived Trophic Factor, HFB1-GDNF, ATF, ATF1, ATF2, HSCR3, HGDNF |
| Accession Number | P39905 |
| Expression System | CHO cell line. Disulfide-linked homodimer. |
| Molecular Weight | 11.6 kDa (monomer, predicted), 16 kDa and 32 kDa (glycosylated, observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively) |
| Sequence | Arg109-Ile211 |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using human neuroblastoma cells. The ED50 for this effect is typically <20 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.05 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Glial Cell-Derived Neurotrophic Factor (GDNF) plays multiple roles in neuronal development, maintenance, and repair. GDNF is produced by astrocytes, oligodendrocytes, Schwann cells, motor neurons, and skeletal muscle to regulate the survival of motor neurons and differentiation dopaminergic neurons during development and to protect against subsequent neurodegeneration. GDNF production in Sertoli cells regulates male germ cell proliferation and differentiation. In cell culture, GDNF is often used to differentiate human pluripotent stem cells (hPSC) progenitors into astrocytes and neurons. GDNF is also used to maintain survival and growth of neurons and astrocytes in culture. Under further culture conditions, GDNF is able to contribute to the maintenance and differentiation of dopaminergic neurons. GDNF signaling takes places by binding to the receptor, GFR alpha1, and forming a complex with RET. |
| Tissue Specificity | GDNF expression has been identified in brain (astrocytes, neurons, substantia nigra), kidney, skeletal muscle, testes, and colon. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of GDNF is associated with Parkinson’s disease, amyotrophic lateral sclerosis (ALS), Huntington’s disease, depression, and drug and alcohol addiction. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0011 |
| Size | 100ug |
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