Recombinant Human GDF-15 Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human GDF-15 Protein |
| Target Symbol | GDF15 |
| Alternative Names | Growth Differentiation Factor 15, GDF15, MIC-1, Macrophage Inhibitory Cytokine 1, NAG1, PDF, PLAB, Prostate Differentiation Factor, PTGFB, PTGF-beta |
| Accession Number | Q99988 |
| Expression System | CHO cell line. Disulfide-linked homodimer. |
| Molecular Weight | 12.5 kDa (monomer, predicted), 12 kDa and 24 kDa (observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively) |
| Sequence | Ala195-Ile308 |
| Purity | >98% under reducing conditions by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by an alkaline phosphatase activity inhibition assay using mouse MC3T3-E1 cells. The ED50 for this effect is typically <2 ng/mL. |
| Formulation | Lyophilized from sterile Tris and NaCl with Trehalose, pH 7.5 |
| Endotoxin Level | <0.05 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Growth Differentiation Factor 15 (GDF-15) is part of the Transforming Growth Factor-beta (TGF-β) superfamily. GDF-15 is a dimeric protein consisting of a pro-domain and a mature, biologically active domain. The mature form of GDF-15 contains a conserved cysteine knot structure typical of the TGF-β family. GDF-15 exerts its biological effects by binding to the GFRAL receptor (GDNF family receptor alpha-like) and activates the RET (rearranged during transfection) kinase signaling pathway. This signaling cascade involves phosphorylation events leading to the activation of MAPK, PI3K, and AKT pathways. GDF-15 has been implicated in the regulation of appetite, energy balance, and tissue repair. It can act as a protective response during stress and is involved in processes like apoptosis, tissue remodeling, and the immune response. |
| Tissue Specificity | GDF-15 expression has been reported in liver tissue, adipose tissue, skeletal muscle cells, cardiac tissue, kidney tissue, brain (hypothalamus and hindbrain) tissue, pancreatic cells, lung tissue, macrophages and dendritic cells. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of GDF-15 has been implicated in tumor progression, metastasis and immune evasion along with cachexia in cancer patients elevated levels of GDF-15 are associated with metabolic abnormalities such as reduced appetite promoting catabolism. Dysregulation of GDF-15 is also associated with atherosclerosis, chronic kidney disease, Alzheimer’s disease, Parkinson’s disease, rheumatoid arthritis, systemic lupus erythematosus (SLE), non-alcoholic fatty liver disease (NAFLD), pre-eclampsia, sarcopenia, and Duchenne muscular dystrophy (DMD). |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0010 |
| Size | 100ug |
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