Recombinant Human G-CSF Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human G-CSF Protein |
| Target Symbol | CSF3 |
| Alternative Names | Granulocyte Colony Stimulating Factor, Colony Stimulating Factor 3GCSF, CSF3, C17orf33, CSF3OS, Filgrastim, Pluripoietin, Lenograstim |
| Accession Number | NP_757373.1 |
| Expression System | E.Coli. |
| Molecular Weight | 18.7 kDa (monomer, predicted) |
| Sequence | Thr31-Pro204 |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using mouse M-NFS-60 cells. The ED50 for this effect is typically 10-50 pg/mL. |
| Formulation | Lyophilized from sterile Sodium Acetate and Sodium Chloride with Trehalose. |
| Endotoxin Level | <0.02 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | G-CSF primarily stimulates the proliferation, differentiation, and survival of neutrophilic granulocyte precursors in the bone marrow and stimulates upregulation of mature neutrophil release into the bloodstream as part of the innate immune response. During an immune response, G-CSF is secreted by monocytes, macrophages, and activated T cells. G-CSF binds to the G-CSF receptor (G-CSFR), which is expressed on the surface of hematopoietic cells, particularly myeloid progenitors. G-CSF signaling occurs via the JAK/STAT, Ras/MAPK and PI3K/Akt pathways. |
| Tissue Specificity | G-CSF expression has been reported in bone marrow stromal cells, fibroblasts, endothelial cells, and epithelial cells. |
| Cellular Localization | Secreted protein |
| Involvement In Disease | Dysregulation of G-CSF is implicated in leukemias (such as acute myeloid leukemia) and myelodysplastic syndromes, autoimmune disorders (rheumatoid arthritis and inflammatory bowel disease), congenital neutropenia, and solid tumors where aberrant expression may contribute to tumor progression, metastasis, and the formation of a supportive tumor microenvironment. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0020UL |
| Size | 100ug |
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