Recombinant Human FGF-basic/FGF2 145aa Protein, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human FGF-basic/FGF2 145aa Protein |
| Target Symbol | FGF2 |
| Alternative Names | Fibroblast Growth Factor basic, BFGF, Basic Fibroblast Growth Factor, Heparin-Binding Growth Factor 2, HBGF2, FGFB, FGF2, Prostatropin |
| Accession Number | NP_001997 |
| Expression System | E.Coli. |
| Molecular Weight | 16.3 kDa (predicted) |
| Sequence | Ala144-Ser288 |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using BALB/c 3T3 mouse fibroblast cells. The ED50 for this effect is typically <0.1 ng/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.05 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Fibroblast Growth Factor Basic (FGF-basic), also known as Fibroblast Growth Factor 2 (FGF-2), displays a diverse range of roles in normal physiological processes, such as proliferation of fibroblasts, endothelial cells and stem cells leading to roles in tissue neovascularization and tissue repair. It promotes the survival and proliferation of neurons, as well as the growth of neuronal processes. FGF-basic has also been implicated in neuroprotection, helping to protect neurons from damage. During embryogenesis, FGF-basic is involved in various developmental processes, including cell differentiation, morphogenesis, and organogenesis. Although FGF-basic is primarily used in stem cell culture to maintain undifferentiated cells, modulation of growth factor supplements in media containing FGF-basic will promote differentiation. FGF-basic is a ligand for FGF Receptors 1-4 (CD331, CD332, CD333, CD334). |
| Tissue Specificity | FGF-basic expression has been identified in endothelial tissue, epithelial tissue, mesenchymal tissue, neuronal tissue and various cancers. |
| Cellular Localization | FGF-basic exists as multiple isoforms that are either initiated with the CUG codon (retained intracellularly) or the AUG codon (secreted forms differing by the removal of a 9aa prosegment). |
| Involvement In Disease | FGF-basic is overexpressed in many types of cancer and is associated with tumor growth, angiogenesis, and metastasis. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0005 |
| Size | 100ug |
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