Recombinant Human EGF Protein, Animal-Free, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Human EGF Protein, Animal-Free |
| Target Symbol | EGF |
| Alternative Names | Epidermal Growth Factor, Beta-Urogastrone, Urogastrone, URG, HOMG4 |
| Accession Number | P01133 |
| Expression System | E. coli using an animal component-free system |
| Molecular Weight | 6.4 kDa (predicted) |
| Sequence | Asn971-Arg1023, with an N-terminal Met |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by a cell proliferation assay using Balb/c-3T3 mouse fibroblast cells. The ED50 for this effect is typically <100 pg/mL. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.01 EU/1ug of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | EGF (Epidermal Growth Factor) is a growth factor that plays several roles related to cell growth, proliferation, and differentiation mediated through binding to its receptor, EGFR (Epidermal Growth Factor Receptor). EGF may be expressed in macrophages, platelets, plasma, milk, urine, saliva, seminal fluid, amniotic fluid and cerebrospinal fluid. Several roles are attributed to EGF such as angiogenesis, epithelial cell growth and maintenance, keratinocyte proliferation, gastrointestinal mucosa integrity, along with neurogenesis and neuronal survival. Due to these wide-ranging functions as a growth factor, recombinant human EGF (rhEGF) is commonly used in cell culture for the maintenance and growth of cell lines, stem cells, and 3-D organoids. |
| Tissue Specificity | EGF expression has been reported in skin, gastrointestinal epithelial tissue, salivary glands, kidney, brain, liver, mammary glands, ovaries and testes. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms. |
| Involvement In Disease | Dysregulation of EGF is associated with several cancers (lung cancer, breast cancer, colorectal cancer, and head and neck cancer), psoriasis, inflammatory bowel disease (IBD), diabetic nephropathy, polycystic kidney disease, peptic ulcers and impaired wound healing. |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0004UL |
| Size | 100ug |
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