Recombinant DNA/RNA Nuclease, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant DNA/RNA Nuclease |
| Target Symbol | NucA |
| Alternative Names | Nuclease A, NucA |
| Accession Number | P13717.2 |
| Expression System | E. coli. |
| Molecular Weight | 28.9 kDa (monomer, predicted) |
| Sequence | Met1-Asn266 |
| Purity | >98-99% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | Determined by its ability to cleave all single or double stranded DNA and RNA similar to the universal nuclease Benzonase®. The specific activity is typically >1.5×106 Unit per mg. |
| Formulation | PBS, pH 7.4, with 50% Glycerol and 2 mM MgCl2 |
| Endotoxin Level | <0.001 EU per 1000 Units (< 1 EU/1000KU) of the protein as determined by the LAL method. |
| Shipping | Shipped on blue ice. Store immediately at -20°C upon receipt. |
| Stability & Storage | 12 months from date of receipt at -20°C. Do not store at -80°C. |
| Target Function | Serratia marcescens Nuclease A (NucA) is a secreted, magnesium-dependent endonuclease that indiscriminately degrades both DNA and RNA, whether single- or double-stranded, linear, circular, or chromosomal, plasmid or viral, into short 2–5-base oligonucleotides. Recombinant NucA, such as Nuclease, has applications in bio-processing to meet guidelines for host cell DNA and viral DNA clearance, lowering lysate viscosity, improving clarification and chromatography, and cleaning up nucleic-acid contamination in cell-free assays. |
| Cellular Localization | Secreted enzyme |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0036UL |
| Size | 100KU |
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