Recombinant Bovine Serum Albumin/BSA Protein, Animal-Free, Active, Low Endotoxin
Description
Product Overview
| Product Name | Recombinant Bovine Serum Albumin/BSA Protein, Animal-Free |
| Target Symbol | ALB |
| Alternative Names | Albumin, BSA |
| Accession Number | A0A140T897 |
| Expression System | Expressed in E. coli using an animal component-free system, with no Fetal Bovine Serum (FBS) |
| Molecular Weight | 66.5 kDa (monomer, predicted) |
| Sequence | Asp25-Ala607 |
| Purity | >98% under reducing conditions by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Activity | The carrier effect of Recombinant Bovine Serum Albumin/BSA was determined by enhancement of cell proliferation using TF-1 human cells. Stout, et al. (2022) Commun Biol 5:466. Cell proliferation in TF-1 human cells was induced in a dose-dependent manner by Recombinant Human IL-3 and demonstrated >25% enhancement in the presence of rBSA. |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
| Endotoxin Level | <0.01 EU/1ug (<10 EU/mg) of the protein as determined by the LAL method. |
| Shipping | Shipped at ambient temperature. |
| Stability & Storage | 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles. |
| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
| Target Function | Bovine serum albumin (BSA) is an approximately 66.5 kDa protein with diverse functions including maintenance of pH and osmotic balance in biochemical assays and culture systems, along with functioning as a carrier protein that binds and transports a wide range of small molecules, including fatty acids, hormones, bilirubin, and metal ions. Additionally, BSA functions as a stabilizer of proteins and enzymes with properties that limit denaturation, aggregation and proteolysis. Due to these functions, recombinant BSA restores many of the transport, protective, and buffering roles lost when serum is removed from chemically defined cell culture media. |
| Tissue Specificity | Plasma |
| Cellular Localization | Secreted protein |
Intended use
Research Use Only
Specifications
| Product information | |
|---|---|
| Catalog number | BEP-0019UL |
| Size | 100ug |
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